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Literature summary extracted from

  • Briozzo, P.; Evrin, C.; Meyer, P.; Assairi, L.; Joly, N.; Barzu, O.; Gilles, A.M.
    Structure of Escherichia coli UMP kinase differs from that of other nucleoside monophosphate kinases and sheds new light on enzyme regulation (2005), J. Biol. Chem., 280, 25533-25540.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.22 GTP
-
 Escherichia coli

Application

EC Number Application Comment Organism
2.7.4.22 drug development as bacterial UMP kinases have no counterpart in eukaryotes, the information provided here can help the design of new antibiotics Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.22 expression in Escherichia coli BL21 (DE3) Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.4.22 bound to the UMP substrate, resolved at 2.3 A resolution, bound to the UDP product, resolved at 2.6 A resolution, bound to UTP, resolved at 2.45 A resolution Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.22 N140A decreases half-denaturation temperature of UMP kinase by around 10°C, moderate loss of sensitivity to UTP inhibition, important loss in activation by GTP Escherichia coli
2.7.4.22 T138A decreases half-denaturation temperature of UMP kinase by around 10°C, results in 4times higher Km for UMP, moderate loss of sensitivity to UTP inhibition, important loss in activation by GTP Escherichia coli
2.7.4.22 T138A/N140A decreases half-denaturation temperature of UMP kinase by around 25°C, increases the apparant Km for ATP and UMP by a factor of 2.6 and 12, respectively Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.22 UTP
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.22 0.05
-
 UMP wild-type Escherichia coli
2.7.4.22 0.056
-
 UMP mutant N140A Escherichia coli
2.7.4.22 0.083
-
 ATP mutant N140A Escherichia coli
2.7.4.22 0.16
-
 ATP wild-type Escherichia coli
2.7.4.22 0.19
-
 UMP mutant T138A Escherichia coli
2.7.4.22 0.29
-
 ATP mutant T138A Escherichia coli
2.7.4.22 0.42
-
 ATP mutant T138A/N140A Escherichia coli
2.7.4.22 0.6
-
 UMP mutant T138A/N140A Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.22 ATP + UMP Escherichia coli
-
 ADP + UDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.22 Escherichia coli P0A7E9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.22
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.22 52
-
 mutant N140A Escherichia coli
2.7.4.22 88
-
 mutant T138A Escherichia coli
2.7.4.22 90
-
 mutant T138A/N140A Escherichia coli
2.7.4.22 96
-
 wild-type Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.22 ATP + UMP
-
 Escherichia coli ADP + UDP
-
 ?
2.7.4.22 ATP + UMP at 30°C, pH 7.4, in the presence of 2 mM MgCl2 Escherichia coli ADP + UDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.22 hexamer crystal structure Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.4.22 UMP kinase
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.22 43
-
 mutant T138A/N140A, temperature of half-inactivation Escherichia coli
2.7.4.22 56
-
 mutant T138A, temperature of half-inactivation Escherichia coli
2.7.4.22 59
-
 mutant N140A, temperature of half-inactivation Escherichia coli
2.7.4.22 68
-
 wild-type, temperature of half-inactivation Escherichia coli